Chrm4 cholinergic receptor, muscarinic 4 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Chrm4provided by MGI
Official Full Name: cholinergic receptor, muscarinic 4provided by MGI
Primary source: MGI:MGI:88399
See related: Ensembl:ENSMUSG00000040495 AllianceGenome:MGI:88399
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: M4; Chrm-4; AChR M4; mm4 mAChR
Summary: Enables G protein-coupled acetylcholine receptor activity. Acts upstream of or within adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway. Located in membrane. Is expressed in several structures, including alimentary system; eye; limb; nervous system; and reproductive system. Orthologous to human CHRM4 (cholinergic receptor muscarinic 4). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in frontal lobe adult (RPKM 11.8), testis adult (RPKM 10.1) and 6 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 E1; 2 50.63 cM

Exon count:: 2

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (91752531..91760180)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (91922189..91929835)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Vagal-mAChR4 signaling promotes Friend virus complex (FV)-induced acute erythroleukemia.
Title: Vagal-mAChR4 signaling promotes Friend virus complex (FV)-induced acute erythroleukemia.
Revealing a compulsive phenotype in cholinergic M4(-/-) mice depends on the inter-trial interval initiation settings in a five choice serial reaction time task.
Title: Revealing a compulsive phenotype in cholinergic M(4)(-/-) mice depends on the inter-trial interval initiation settings in a five choice serial reaction time task.
Lack of M4 muscarinic receptors in the striatum, thalamus and intergeniculate leaflet alters the biological rhythm of locomotor activity in mice.
Title: Lack of M(4) muscarinic receptors in the striatum, thalamus and intergeniculate leaflet alters the biological rhythm of locomotor activity in mice.
The Firing of Theta State-Related Septal Cholinergic Neurons Disrupt Hippocampal Ripple Oscillations via Muscarinic Receptors.
Title: The Firing of Theta State-Related Septal Cholinergic Neurons Disrupt Hippocampal Ripple Oscillations via Muscarinic Receptors.
Muscarinic acetylcholine receptor regulates self-renewal of early erythroid progenitors.
Title: Muscarinic acetylcholine receptor regulates self-renewal of early erythroid progenitors.
Muscarinic receptors (MR) are typical members of G protein-coupled receptors and can be divided into five subtypes (M1-M5), which activate different G proteins.
Title: The deletion of M(4) muscarinic receptors increases motor activity in females in the dark phase.
The findings of this study indicated that the cocaine-like S(D) effects of muscarinic antagonists are primarily mediated through M1 receptors, with a minor contribution of M4 receptors.
Title: Effects of muscarinic receptor antagonists on cocaine discrimination in wild-type mice and in muscarinic receptor M(1), M(2), and M(4) receptor knockout mice.
The three receptor sets considered (mAChR, AR and TrkB receptors) intervene in modulating the conditions of the competition between nerve endings.
Title: Presynaptic muscarinic acetylcholine autoreceptors (M1, M2 and M4 subtypes), adenosine receptors (A1 and A2A) and tropomyosin-related kinase B receptor (TrkB) modulate the developmental synapse elimination process at the neuromuscular junction.
In the present study, the functional coupling of Gi/o protein-coupled receptors to GalR1, and the CB1 receptor subtype for endocannabinoids were analyzed in the 3xTg-AD mice model of Alzheimer's disease in the prodromal and advanced stages. In addition, the activity mediated by Gi/o protein-coupled M2/4 muscarinic receptor subtypes was also analyzed in brain areas involved in anxiety and cognition.
Title: Activity of muscarinic, galanin and cannabinoid receptors in the prodromal and advanced stages in the triple transgenic mice model of Alzheimer's disease.
Muscarinic acetylcholine receptor subtype 4 is essential for cholinergic stimulation of duodenal bicarbonate secretion in mice - relationship to D cell/somatostatin
Title: Muscarinic acetylcholine receptor subtype 4 is essential for cholinergic stimulation of duodenal bicarbonate secretion in mice - relationship to D cell/somatostatin.

Submit: New GeneRIF Correction See all GeneRIFs (31)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1) 1 citation
Targeted (5) 1 citation
Endonuclease-mediated (4)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

UniSTS:492564 (e-PCR)
- UniSTS:492564

Chrm4 (e-PCR), detects polymorphism
- UniSTS:141339

UniSTS:481077 (e-PCR)
- UniSTS:481077

Chrm4 (e-PCR), detects polymorphism
- UniSTS:506798

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables G protein-coupled acetylcholine receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables G protein-coupled acetylcholine receptor activity	IDA Inferred from Direct Assay more info	PubMed
enables G protein-coupled receptor activity	IEA Inferred from Electronic Annotation more info
enables G protein-coupled serotonin receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables guanyl-nucleotide exchange factor activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger	IBA Inferred from Biological aspect of Ancestor more info
involved_in adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in chemical synaptic transmission	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of locomotion	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in asymmetric synapse	ISO Inferred from Sequence Orthology more info
located_in axon terminus	ISO Inferred from Sequence Orthology more info
is_active_in dendrite	IBA Inferred from Biological aspect of Ancestor more info
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
is_active_in postsynaptic density membrane	ISO Inferred from Sequence Orthology more info
located_in postsynaptic membrane	IEA Inferred from Electronic Annotation more info
is_active_in presynaptic membrane	ISO Inferred from Sequence Orthology more info
colocalizes_with sarcolemma	ISO Inferred from Sequence Orthology more info
is_active_in synapse	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: muscarinic acetylcholine receptor M4

Names: muscarinic acetylcholine receptor 4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001416938.1 → NP_001403867.1 muscarinic acetylcholine receptor M4

Status: VALIDATED

Source sequence(s)

AL731772

UniProtKB/Swiss-Prot

P32211, Q64056

UniProtKB/TrEMBL

Q0VBU3
NM_007699.3 → NP_031725.1 muscarinic acetylcholine receptor M4

See identical proteins and their annotated locations for NP_031725.1

Status: VALIDATED

Source sequence(s)

AL731772

Consensus CDS

CCDS16439.1

UniProtKB/Swiss-Prot

P32211, Q64056

UniProtKB/TrEMBL

Q0VBU3

Related

ENSMUSP00000040808.4, ENSMUST00000045537.4

Conserved Domains (2) summary

pfam00001
Location:48 → 453

7tm_1; 7 transmembrane receptor (rhodopsin family)

cl21561
Location:40 → 172

7tm_4; Olfactory receptor