Cd4 CD4 antigen [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Cd4provided by MGI
Official Full Name: CD4 antigenprovided by MGI
Primary source: MGI:MGI:88335
See related: Ensembl:ENSMUSG00000023274 AllianceGenome:MGI:88335
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: L3T4; Ly-4
Summary: Predicted to enable several functions, including interleukin-16 binding activity; interleukin-16 receptor activity; and protein homodimerization activity. Involved in helper T cell enhancement of adaptive immune response. Acts upstream of or within several processes, including defense response to Gram-negative bacterium; positive regulation of calcium-mediated signaling; and positive regulation of peptidyl-tyrosine phosphorylation. Located in endoplasmic reticulum; external side of plasma membrane; and membrane raft. Is expressed in several structures, including alimentary system; brain; genitourinary system; hemolymphoid system; and liver and biliary system. Used to study type 1 diabetes mellitus. Human ortholog(s) of this gene implicated in immunodeficiency 79. Orthologous to human CD4 (CD4 molecule). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in thymus adult (RPKM 167.0), spleen adult (RPKM 15.0) and 1 other tissue See more
Orthologs: human all
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Genomic context

Location:: 6 F2; 6 59.17 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	6	NC_000072.7 (124841656..124865210, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	6	NC_000072.6 (124864693..124888247, complement)

Chromosome 6 - NC_000072.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A Cryptosporidium parvum vaccine candidate effect on immunohistochemical profiling of CD4[+], CD8[+], Caspase-3 and NF-kappaB in mice.
Title: A Cryptosporidium parvum vaccine candidate effect on immunohistochemical profiling of CD4(+), CD8(+), Caspase-3 and NF-κB in mice.
ThPOK is a critical multifaceted regulator of myeloid lineage development.
Title: ThPOK is a critical multifaceted regulator of myeloid lineage development.
Transfer of mesenchymal stem cell mitochondria to CD4[+] T cells contributes to repress Th1 differentiation by downregulating T-bet expression.
Title: Transfer of mesenchymal stem cell mitochondria to CD4(+) T cells contributes to repress Th1 differentiation by downregulating T-bet expression.
CD4(+)/IL4(+) lymphocytes of the lamina propria and substance P promote colonic protection during acute stress.
Title: CD4(+)/IL‑4(+) lymphocytes of the lamina propria and substance P promote colonic protection during acute stress.
Bhlhe40 is a key transcription factor that regulates CD4+ T cell-mediated GM-CSF production and pathological damage in the colon in graft-versus-host disease. Bhlhe40 is a key transcription factor that regulates CD4+ T cell-mediated GM-CSF production and pathological damage in the colon in graft-versus-host disease.
Title: Pathogenic Bhlhe40+ GM-CSF+ CD4+ T cells promote indirect alloantigen presentation in the GI tract during GVHD.
CAF-1 and the DNA methyltransferases cooperate to maintain Cd4 silencing and thereby contribute to CD8+ T-cell function and lineage integrity
Title: The histone chaperone CAF-1 cooperates with the DNA methyltransferases to maintain Cd4 silencing in cytotoxic T cells.
Induction of CD4(+)CD25(+)FOXP3(+) regulatory T cells by mesenchymal stem cells is associated with modulation of ubiquitination factors and Treg-specific demethylated region demethylation.
Title: Induction of CD4(+)CD25(+)FOXP3(+) regulatory T cells by mesenchymal stem cells is associated with modulation of ubiquitination factors and TSDR demethylation.
IL-27 induces IL-10 production in CD4 T cells during mouse cytomegalovirus infection.
Title: IL-27 regulates the number, function and cytotoxic program of antiviral CD4 T cells and promotes cytomegalovirus persistence.
Enhancers that regulate Cd4 expression perform multiple functions, including not only direct support of transcriptional activity, but also regulation of the gene's methylation state and entrainment of cis-elements that sustain expression after the cells exit the thymus.
Title: Stage-specific epigenetic regulation of CD4 expression by coordinated enhancer elements during T cell development.
Study identified the maturation enhancer E4m, validated its role in regulating CD4 expression, and isolate Bcl11b as an important activator for E4m.
Title: Runx-dependent and silencer-independent repression of a maturation enhancer in the Cd4 gene.

Submit: New GeneRIF Correction See all GeneRIFs (136)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Spontaneous (3) 1 citation
Targeted (12) 1 citation
Chemically induced (ENU) (5)
Endonuclease-mediated (14) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

X04836 (e-PCR)
- UniSTS:159572

Cd4 (e-PCR), detects polymorphism
- UniSTS:141282

Cd4 (e-PCR), detects polymorphism
- UniSTS:141283

D6Mit24 (e-PCR), detects polymorphism
- UniSTS:130787

Cd4 (e-PCR), detects polymorphism
- UniSTS:141284

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables MHC class II protein binding	IBA Inferred from Biological aspect of Ancestor more info
enables MHC class II protein binding	ISO Inferred from Sequence Orthology more info
enables MHC class II protein complex binding	ISO Inferred from Sequence Orthology more info
enables coreceptor activity	IEA Inferred from Electronic Annotation more info
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables immunoglobulin binding	ISO Inferred from Sequence Orthology more info
enables interleukin-16 binding	ISO Inferred from Sequence Orthology more info
enables interleukin-16 receptor activity	ISO Inferred from Sequence Orthology more info
enables lipid binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables protein kinase binding	ISO Inferred from Sequence Orthology more info
enables protein tyrosine kinase binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein tyrosine kinase binding	ISO Inferred from Sequence Orthology more info
enables signaling receptor binding	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in T cell activation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of T cell activation	IDA Inferred from Direct Assay more info	PubMed
involved_in T cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in T cell differentiation	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in T cell selection	ISO Inferred from Sequence Orthology more info
involved_in T cell selection	ISS Inferred from Sequence or Structural Similarity more info	PubMed
acts_upstream_of_or_within adaptive immune response	IEA Inferred from Electronic Annotation more info
acts_upstream_of calcium-mediated signaling	IDA Inferred from Direct Assay more info	PubMed
involved_in cell adhesion	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cell surface receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in cell surface receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to granulocyte macrophage colony-stimulating factor stimulus	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cytokine production	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within cytokine production	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within defense response to Gram-negative bacterium	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in helper T cell enhancement of adaptive immune response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in immune response	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within immune system process	IEA Inferred from Electronic Annotation more info
involved_in interleukin-15-mediated signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in interleukin-15-mediated signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in macrophage differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within maintenance of protein location in cell	ISO Inferred from Sequence Orthology more info	PubMed
acts_upstream_of peptidyl-tyrosine phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of ERK1 and ERK2 cascade	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of ERK1 and ERK2 cascade	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of MAPK cascade	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of T cell activation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of T cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of calcium ion transport into cytosol	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of calcium-mediated signaling	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of canonical NF-kappaB signal transduction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of kinase activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of monocyte differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of peptidyl-tyrosine phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of protein kinase activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of viral entry into host cell	ISO Inferred from Sequence Orthology more info
involved_in regulation of T cell activation	ISO Inferred from Sequence Orthology more info
involved_in regulation of calcium ion transport	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cell surface	IDA Inferred from Direct Assay more info	PubMed
located_in cell surface	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum lumen	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in external side of plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in external side of plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in external side of plasma membrane	ISO Inferred from Sequence Orthology more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in membrane raft	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane raft	IDA Inferred from Direct Assay more info	PubMed
located_in membrane raft	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: T-cell surface glycoprotein CD4

Names: T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_013488.3 → NP_038516.1 T-cell surface glycoprotein CD4 precursor

See identical proteins and their annotated locations for NP_038516.1

Status: VALIDATED

Source sequence(s)

AK153707, AK161775, BX639790

Consensus CDS

CCDS20535.1

UniProtKB/Swiss-Prot

P06332

UniProtKB/TrEMBL

Q3TSV7, Q61396

Related

ENSMUSP00000024044.7, ENSMUST00000024044.7

Conserved Domains (5) summary

smart00410
Location:131 → 205

IG_like; Immunoglobulin like

pfam05790
Location:128 → 207

C2-set; Immunoglobulin C2-set domain

pfam09191
Location:212 → 316

CD4-extracel; CD4, extracellular

pfam12104
Location:425 → 452

Tcell_CD4_C; T cell CD4 receptor C terminal region

cl11960
Location:29 → 124

Ig; Immunoglobulin domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000072.7 Reference GRCm39 C57BL/6J

Range

124841656..124865210 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006505462.2 → XP_006505525.1 T-cell surface glycoprotein CD4 isoform X2

UniProtKB/TrEMBL

Q61396

Conserved Domains (3) summary

pfam05790
Location:317 → 387

C2-set; Immunoglobulin C2-set domain

pfam09191
Location:212 → 316

CD4-extracel; CD4, extracellular

cl11960
Location:29 → 124

Ig; Immunoglobulin domain
XM_006505461.4 → XP_006505524.1 T-cell surface glycoprotein CD4 isoform X1

UniProtKB/TrEMBL

Q61396

Conserved Domains (3) summary

pfam05790
Location:317 → 387

C2-set; Immunoglobulin C2-set domain

pfam09191
Location:212 → 316

CD4-extracel; CD4, extracellular

cl11960
Location:29 → 124

Ig; Immunoglobulin domain