Slc25a20 solute carrier family 25 member 20 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Slc25a20provided by RGD
Official Full Name: solute carrier family 25 member 20provided by RGD
Primary source: RGD:621443
See related: Ensembl:ENSRNOG00000020288 AllianceGenome:RGD:621443
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: CACT
Summary: Enables acyl carnitine transmembrane transporter activity. Involved in carnitine transmembrane transport. Predicted to be located in cytosol and mitochondrion. Human ortholog(s) of this gene implicated in carnitine-acylcarnitine translocase deficiency. Orthologous to human SLC25A20 (solute carrier family 25 member 20). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Heart (RPKM 822.9), Liver (RPKM 499.8) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 8q32

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	8	NC_086026.1 (118243573..118265027)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	8	NC_051343.1 (109365056..109386512)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	8	NC_005107.4 (117455308..117476762)

Chromosome 8 - NC_086026.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Inhibition by the NO-releasing compound GSNO suggests that C136 S-nitrosylation of native and recombinant CACT may have a role in the regulation of the beta-oxidation pathway.
Title: Nitric oxide inhibits the mitochondrial carnitine/acylcarnitine carrier through reversible S-nitrosylation of cysteine 136.
The experimental data indicate that acetylation of CACT inhibits its transport activity, and thus may contribute to the regulation of the mitochondrial beta-oxidation pathway.
Title: Post-translational modification by acetylation regulates the mitochondrial carnitine/acylcarnitine transport protein.
Report interaction between carnitine palmitoyltransferase 2 and carnitine/acylcarnitine translocase supporting the channeling of acylcarnitines and carnitine at level of the inner mitochondrial membrane.
Title: Carnitine/acylcarnitine translocase and carnitine palmitoyltransferase 2 form a complex in the inner mitochondrial membrane.
The data demonstrated that V25, P78, V82, M85 and C89 are involved in the acyl chain binding to the CAC in c-state.
Title: Identification by site-directed mutagenesis of a hydrophobic binding site of the mitochondrial carnitine/acylcarnitine carrier involved in the interaction with acyl groups.
Site-directed mutagenesis and chemical modification of the six native cysteine residues: implications for the role of cysteine-136
Title: Site-directed mutagenesis and chemical modification of the six native cysteine residues of the rat mitochondrial carnitine carrier: implications for the role of cysteine-136.
Cys(58), Cys(136), and Cys(155) become close in the tertiary structure of the CAC during its catalytic cycle.
Title: Identification by site-directed mutagenesis and chemical modification of three vicinal cysteine residues in rat mitochondrial carnitine/acylcarnitine transporter.

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General gene information

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Markers

RH143998 (e-PCR)
- UniSTS:233244

BE106540 (e-PCR)
- UniSTS:246308

RH136924 (e-PCR)
- UniSTS:219013

Slc25a20 (e-PCR), detects polymorphism
- UniSTS:547043

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables acyl carnitine transmembrane transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables acyl carnitine transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables acyl carnitine transmembrane transporter activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in acyl carnitine transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in carnitine transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in carnitine transmembrane transport	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within in utero embryonic development	ISO Inferred from Sequence Orthology more info
involved_in lipid transport	IEA Inferred from Electronic Annotation more info
involved_in mitochondrial transport	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in mitochondrial envelope	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrial inner membrane	TAS Traceable Author Statement more info	PubMed
located_in mitochondrion	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: mitochondrial carnitine/acylcarnitine carrier protein

Names: CAC; carnitine/acylcarnitine translocase; solute carrier family 25 (carnitine/acylcarnitine translocase), member 20; solute carrier family 25 (mitochondrial carnitine/acylcarnitine translocase), member 20

NP_446417.2

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_053965.2 → NP_446417.2 mitochondrial carnitine/acylcarnitine carrier protein

See identical proteins and their annotated locations for NP_446417.2

Status: PROVISIONAL

Source sequence(s)

BC081749

UniProtKB/Swiss-Prot

P97521

UniProtKB/TrEMBL

A6I375, F7F1T4, Q66HP8

Related

ENSRNOP00000027520.2, ENSRNOT00000027520.5

Conserved Domains (2) summary

PTZ00169
Location:11 → 277

PTZ00169; ADP/ATP transporter on adenylate translocase; Provisional

pfam00153
Location:106 → 198

Mito_carr; Mitochondrial carrier protein