Plce1 phospholipase C, epsilon 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Plce1provided by RGD
Official Full Name: phospholipase C, epsilon 1provided by RGD
Primary source: RGD:69424
See related: Ensembl:ENSRNOG00000014276 AllianceGenome:RGD:69424
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Plce
Summary: Enables phosphatidylinositol phospholipase C activity and small GTPase binding activity. Involved in G protein-coupled receptor signaling pathway and Ras protein signal transduction. Located in cytoplasm. Human ortholog(s) of this gene implicated in nephrotic syndrome; nephrotic syndrome type 2; and nephrotic syndrome type 3. Orthologous to human PLCE1 (phospholipase C epsilon 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Heart (RPKM 96.2), Lung (RPKM 62.6) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 1q53

Exon count:: 36

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	1	NC_086019.1 (245655855..245964966)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	1	NC_051336.1 (236243445..236552571)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	1	NC_005100.4 (257156023..257465440)

Chromosome 1 - NC_086019.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

PLCepsilon1 mediates one-lung ventilation injury by regulating the p38/RhoA/NFkappaB activation loop.
Title: PLCε1 mediates one-lung ventilation injury by regulating the p38/RhoA/NFκB activation loop.
Structure of phospholipase Cepsilon reveals an integrated RA1 domain and previously unidentified regulatory elements.
Title: Structure of phospholipase Cε reveals an integrated RA1 domain and previously unidentified regulatory elements.
PLCE1 promotes myocardial ischemia-reperfusion injury in H/R H9c2 cells and I/R rats by promoting inflammation.
Title: PLCE1 promotes myocardial ischemia-reperfusion injury in H/R H9c2 cells and I/R rats by promoting inflammation.
The authors demonstrate that endogenous beta1-adrenergic receptors at the Golgi apparatus in cardiac myocytes are required to stimulate hypertrophic Epac/PLCe-dependent phosphatidylinositol 4-phosphate hydrolysis at the Golgi, and that these intracellular beta1-adrenergic receptors can be accessed by physiological neurotransmitters, and synthetic b-blockers and agonists.
Title: Golgi localized β1-adrenergic receptors stimulate Golgi PI4P hydrolysis by PLCε to regulate cardiac hypertrophy.
These results reveal distinct pools of cyclic nucleotides that either inhibit PLCepsilon at the Golgi through PKA/PKG, or activate PLCepsilon at the Golgi through Epac. These data together reveal a new mechanism by which ANF and selective PDE inhibitors can protect against cardiac hypertrophy.
Title: Compartmentalized cyclic nucleotides have opposing effects on regulation of hypertrophic phospholipase Cε signaling in cardiac myocytes.
These results provide first evidence that Gbetagamma directly interacts with PLC and yield insights into the mechanism by which betagamma subunits activate PLC.
Title: G protein βγ subunits directly interact with and activate phospholipase Cϵ.
results have shown a novel role of PLCepsilon in the maintenance of endothelial barrier function, via its CDC25 GEF domain and lipase activity, and subsequent up-regulation of Rap1 activity
Title: Phospholipase Cε Modulates Rap1 Activity and the Endothelial Barrier.
Phospholipase C epsilon scaffolds to muscle-specific A kinase anchoring protein (mAKAPbeta) and integrates multiple hypertrophic stimuli in cardiac myocytes.
Title: Phospholipase C epsilon scaffolds to muscle-specific A kinase anchoring protein (mAKAPbeta) and integrates multiple hypertrophic stimuli in cardiac myocytes.
PLC-epsilon can be directly and concomitantly activated by both RhoA and individual Ras GTPases resulting in diverse upstream control of signaling cascades downstream of PLC-epsilon
Title: Dual activation of phospholipase C-epsilon by Rho and Ras GTPases.
PLC epsilon down-regulates regulation of integrin activation via an independent H-ras signaling pathway.
Title: Phospholipase C epsilon suppresses integrin activation.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH138895 (e-PCR)
- UniSTS:220982

SEPHS1_9288 (e-PCR)
- UniSTS:467877

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables enzyme binding	ISS Inferred from Sequence or Structural Similarity more info
enables guanyl-nucleotide exchange factor activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables phosphatidylinositol phospholipase C activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphatidylinositol phospholipase C activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol phospholipase C activity	IEA Inferred from Electronic Annotation more info
enables phosphatidylinositol phospholipase C activity	ISO Inferred from Sequence Orthology more info
enables phosphatidylinositol phospholipase C activity	ISS Inferred from Sequence or Structural Similarity more info
enables phospholipase C activity	ISO Inferred from Sequence Orthology more info
enables phospholipase C activity	ISS Inferred from Sequence or Structural Similarity more info
enables small GTPase binding	IDA Inferred from Direct Assay more info	PubMed
enables small GTPase binding	IEA Inferred from Electronic Annotation more info
enables small GTPase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in G protein-coupled receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in G protein-coupled receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in Ras protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in Ras protein signal transduction	IDA Inferred from Direct Assay more info	PubMed
involved_in Ras protein signal transduction	IEA Inferred from Electronic Annotation more info
involved_in Ras protein signal transduction	ISO Inferred from Sequence Orthology more info
involved_in diacylglycerol biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in diacylglycerol biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in diacylglycerol biosynthetic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in epidermal growth factor receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in epidermal growth factor receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in epidermal growth factor receptor signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in glomerulus development	IEA Inferred from Electronic Annotation more info
involved_in glomerulus development	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	ISS Inferred from Sequence or Structural Similarity more info
involved_in lipid catabolic process	IEA Inferred from Electronic Annotation more info
involved_in phosphatidylinositol metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in phosphatidylinositol-mediated signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in phospholipase C-activating G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in phospholipase C-activating G protein-coupled receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in phospholipase C-activating G protein-coupled receptor signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of lamellipodium assembly	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of lamellipodium assembly	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of lamellipodium assembly	ISS Inferred from Sequence or Structural Similarity more info
involved_in release of sequestered calcium ion into cytosol	IBA Inferred from Biological aspect of Ancestor more info
involved_in small GTPase-mediated signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in Golgi membrane	IEA Inferred from Electronic Annotation more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISS Inferred from Sequence or Structural Similarity more info
located_in lamellipodium	IEA Inferred from Electronic Annotation more info
located_in lamellipodium	ISO Inferred from Sequence Orthology more info
located_in lamellipodium	ISS Inferred from Sequence or Structural Similarity more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1

Names: PLC-epsilon-1; phosphoinositide phospholipase C-epsilon-1; phosphoinositide-specific phospholipase C epsilon-1; phospholipase C epsilon

NP_446210.1

EC 3.1.4.11

XP_038964220.1

EC 3.1.4.11

XP_063118876.1

EC 3.1.4.11

XP_063118888.1

EC 3.1.4.11

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_053758.2 → NP_446210.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1

See identical proteins and their annotated locations for NP_446210.1

Status: PROVISIONAL

Source sequence(s)

AF323615

UniProtKB/Swiss-Prot

Q99P84

UniProtKB/TrEMBL

A0A0G2K2A6

Related

ENSRNOP00000062667.1, ENSRNOT00000067149.4

Conserved Domains (6) summary

cd00275
Location:1847 → 1963

C2_PLC_like; C2 domain present in Phosphoinositide-specific phospholipases C (PLC)

cd01780
Location:2116 → 2215

PLC_epsilon_RA; Ubiquitin-like domain of Phosphatidylinositide-specific phospholipase

cd08596
Location:1372 → 1812

PI-PLCc_epsilon; Catalytic domain of metazoan phosphoinositide-specific phospholipase C-epsilon

PLN02952
Location:1299 → 1962

PLN02952; phosphoinositide phospholipase C

pfam00617
Location:531 → 710

RasGEF; RasGEF domain

pfam09279
Location:1309 → 1360

EF-hand_like; Phosphoinositide-specific phospholipase C, efhand-like

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086019.1 Reference GRCr8

Range

245655855..245964966

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039108292.2 → XP_038964220.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1

UniProtKB/TrEMBL

A0A0G2K2A6, A6I190, G3V9X9

Conserved Domains (6) summary

cd00275
Location:1847 → 1963

C2_PLC_like; C2 domain present in Phosphoinositide-specific phospholipases C (PLC)

cd08596
Location:1372 → 1812

PI-PLCc_epsilon; Catalytic domain of metazoan phosphoinositide-specific phospholipase C-epsilon

cd16203
Location:1179 → 1359

EFh_PI-PLCepsilon; EF-hand motif found in phosphoinositide phospholipase C epsilon 1 (PI-PLC-epsilon1)

cd01780
Location:2113 → 2215

RA2_PLC-epsilon; Ras-associating (RA) domain 2 found in Phosphatidylinositide-specific phospholipase C (PLC)-epsilon

cd17229
Location:1986 → 2093

RA1_PLC-epsilon; Ras-associating (RA) domain 1 found in Phosphatidylinositide-specific phospholipase C (PLC)-epsilon

pfam00617
Location:531 → 710

RasGEF; RasGEF domain
XM_063262806.1 → XP_063118876.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X2
XM_063262818.1 → XP_063118888.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X3