U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

USP20 ubiquitin specific peptidase 20 [ Homo sapiens (human) ]

Gene ID: 10868, updated on 5-Mar-2024

Summary

Go to the top of the page Help
Official Symbol
USP20provided by HGNC
Official Full Name
ubiquitin specific peptidase 20provided by HGNC
Primary source
HGNC:HGNC:12619
See related
Ensembl:ENSG00000136878 MIM:615143; AllianceGenome:HGNC:12619
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
VDU2; hVDU2; LSFR3A
Summary
This gene encodes a ubiquitin specific processing protease that was first identified as a substrate of the VHL (von Hippel-Lindau disease) protein E3 ubiquitin ligase complex. In addition to being ubiquitinated by the VHL-E3 ligase complex, this enzyme deubiquitinates hypoxia-inducible factor (HIF)-1 alpha and thereby causes increased expression of HIF-1alpha targeted genes which play a role in angiogenesis, glucose metabolism, cell proliferation and metastasis. The enzyme encoded by this gene also regulates G-protein coupled receptor signaling by mediating the deubiquitination of beta-2 adrenergic receptor (ADRB2). This enzyme is a ubiquitously expressed thiolester hydrolase. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jan 2013]
Expression
Ubiquitous expression in lymph node (RPKM 8.9), bone marrow (RPKM 8.3) and 25 other tissues See more
Orthologs
mouse all
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
See USP20 in Genome Data Viewer
Location:
9q34.11
Exon count:
26
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 9 NC_000009.12 (129835458..129881828)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 9 NC_060933.1 (142040029..142086556)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 9 NC_000009.11 (132597737..132644107)

Chromosome 9 - NC_000009.12Genomic Context describing neighboring genes Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 20390 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29122 Neighboring gene Sharpr-MPRA regulatory region 5710 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 20391 Neighboring gene H3K27ac hESC enhancer GRCh37_chr9:132597987-132598487 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr9:132601680-132602180 Neighboring gene torsin family 1 member A Neighboring gene chromosome 9 open reading frame 78 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29123 Neighboring gene Sharpr-MPRA regulatory region 12255 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr9:132632499-132632998 Neighboring gene H3K27ac hESC enhancer GRCh37_chr9:132636644-132637144 Neighboring gene Sharpr-MPRA regulatory region 12063 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr9:132647083-132647764 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29125 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 20393 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29126 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr9:132651249-132652136 Neighboring gene microRNA 6855 Neighboring gene Sharpr-MPRA regulatory region 3297 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29128 Neighboring gene Sharpr-MPRA regulatory region 1290 Neighboring gene formin binding protein 1 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29129 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29130 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29131 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29132 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 20394 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29133 Neighboring gene Sharpr-MPRA regulatory region 9235 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29134 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29135 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29136 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29137 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29138 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29139 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29140 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29141 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29142 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29143 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29144 Neighboring gene ReSE screen-validated silencer GRCh37_chr9:132786300-132786450 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29145 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29146 Neighboring gene H3K27ac hESC enhancer GRCh37_chr9:132796970-132797470 Neighboring gene H3K27ac hESC enhancer GRCh37_chr9:132797471-132797971 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29148 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 20395 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 20396 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 20397 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr9:132811133-132811633 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 20398 Neighboring gene G protein-coupled receptor 107 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr9:132878169-132878714 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr9:132881191-132882139 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 20399 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29149

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help
  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. Expression, purification and characterization of the second DUSP domain of deubiquitinase USP20/VDU2. Hu S, et al. Protein Expr Purif, 2021 May. PMID 33529762
  2. Deubiquitinase USP20 promotes breast cancer metastasis by stabilizing SNAI2. Li W, et al. Genes Dev, 2020 Oct 1. PMID 32943575, Free PMC Article
  3. USP20 positively regulates tumorigenesis and chemoresistance through β-catenin stabilization. Wu C, et al. Cell Death Differ, 2018 Nov. PMID 29867130, Free PMC Article
  4. USP20 regulates the stability of EMT transcription factor SOX4 and influences colorectal cancer metastasis. Guan Y, et al. Pathol Res Pract, 2022 May. PMID 35405623
  5. Deubiquitinases USP20/33 promote the biogenesis of tail-anchored membrane proteins. Culver JA, et al. J Cell Biol, 2021 May 3. PMID 33792613, Free PMC Article

See all (66) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Phosphorylation of USP20 on Ser334 by IRAK1 promotes IL-1beta-evoked signaling in vascular smooth muscle cells and vascular inflammation.
    Title: Phosphorylation of USP20 on Ser334 by IRAK1 promotes IL-1β-evoked signaling in vascular smooth muscle cells and vascular inflammation.
  2. Expression, purification and characterization of the second DUSP domain of deubiquitinase USP20/VDU2.
    Title: Expression, purification and characterization of the second DUSP domain of deubiquitinase USP20/VDU2.
  3. Deubiquitinases USP20/33 promote the biogenesis of tail-anchored membrane proteins.
    Title: Deubiquitinases USP20/33 promote the biogenesis of tail-anchored membrane proteins.
  4. Deubiquitinase USP20 promotes breast cancer metastasis by stabilizing SNAI2.
    Title: Deubiquitinase USP20 promotes breast cancer metastasis by stabilizing SNAI2.
  5. The Deubiquitinating Enzyme USP20 Regulates the TNFalpha-Induced NF-kappaB Signaling Pathway through Stabilization of p62.
    Title: The Deubiquitinating Enzyme USP20 Regulates the TNFα-Induced NF-κB Signaling Pathway through Stabilization of p62.
  6. Study revealed the molecular basis for the weak binding of USP20 N-terminal zinc-finger ubiquitin binding domain (ZnF-UBP) to mono-ubiquitin and poly-ubiquitin. The solution structure of USP20 ZnF-UBP domain consists of a central beta-sheet with either one alpha-helix or two alpha-helices packed on each side of the sheet. The overall structure of USP20 ZnF-UBP domain is stabilized by three coordinated zinc ions.
    Title: Structural and functional studies of USP20 ZnF-UBP domain by NMR.
  7. USP20 is a positive regulator of beta-catenin in cancer cell migration, invasion and tumor growth in vivo. Mechanistically, USP20 deubiquitinates and stabilizes beta-catenin and is a potential biomarker for predicting chemoresistance in cancer.
    Title: USP20 positively regulates tumorigenesis and chemoresistance through β-catenin stabilization.
  8. our results demonstrate a role of the USP20 deubiquitinating enzyme in acting as a critical regulator of ULK1 and show a novel mechanism of the autophagy process in terms of ULK1 stability.
    Title: The deubiquitinating enzyme USP20 stabilizes ULK1 and promotes autophagy initiation.
  9. USP20 regulates actin cytoskeleton organization and cell migration in a manner dependent on ERK3 expression. The results identify USP20 as a bona fide regulator of ERK3 stability and physiological functions.
    Title: Deubiquitinating Enzyme USP20 Regulates Extracellular Signal-Regulated Kinase 3 Stability and Biological Activity.
  10. we investigated the inter-action between PKM2 and USP20. Our results suggest a new molecular pathway in cancer metabolism through the regulation of PKM2.
    Title: Regulation of pyruvate kinase isozyme M2 is mediated by the ubiquitin-specific protease 20.
Submit: New GeneRIF Correction See all GeneRIFs (18)

Phenotypes

Go to the top of the page Help

Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

Go to the top of the page Help

See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

HIV-1 interactions

Go to the top of the page Help

Replication interactions

Interaction Pubs
Knockdown of ubiquitin specific peptidase 20 (USP20) by siRNA inhibits HIV-1 replication in HeLa P4/R5 cells PubMed

Protein interactions

Protein Gene Interaction Pubs
Tat tat Expression of HIV-1 Tat downregulates the abundance of ubiquitin specific peptidase 20 (USP20) in the nucleoli of Jurkat T-cells PubMed

Go to the HIV-1, Human Interaction Database

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Markers

Homology

Clone Names

  • KIAA1003

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables G protein-coupled receptor binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables cysteine-type deubiquitinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables cysteine-type deubiquitinase activity TAS
Traceable Author Statement
more info
  
enables cysteine-type endopeptidase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in antiviral innate immune response IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in endocytosis IEA
Inferred from Electronic Annotation
more info
  
involved_in negative regulation of canonical NF-kappaB signal transduction IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of autophagy IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein K48-linked deubiquitination IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein K63-linked deubiquitination IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein deubiquitination IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in proteolysis IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of G protein-coupled receptor signaling pathway IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in centrosome IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in cytoplasm IC
Inferred by Curator
more info
 PubMed 
located_in cytosol TAS
Traceable Author Statement
more info
  
located_in endoplasmic reticulum IEA
Inferred from Electronic Annotation
more info
  
located_in perinuclear region of cytoplasm IEA
Inferred from Electronic Annotation
more info
  

General protein information

Go to the top of the page Help
Preferred Names
ubiquitin carboxyl-terminal hydrolase 20
Names
deubiquitinating enzyme 20
pVHL-interacting deubiquitinating enzyme 2
ubiquitin thioesterase 20
ubiquitin thiolesterase 20
ubiquitin-specific-processing protease 20
NP_001008563.2
NP_001103773.2
NP_006667.3

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_033097.2 RefSeqGene

    Range
    5030..51400
    Download
    GenBank, FASTA, Sequence Viewer (Graphics), LRG_711

mRNA and Protein(s)

  1. NM_001008563.5 → NP_001008563.2  ubiquitin carboxyl-terminal hydrolase 20

    See identical proteins and their annotated locations for NP_001008563.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) has different 5' and 3' non-coding, terminal exons, compared to transcript variant 1. All three transcript variants encode the same protein.
    Source sequence(s)
    AL158207
    Consensus CDS
    CCDS43892.1
    UniProtKB/Swiss-Prot
    Q541F1, Q8IXQ1, Q96LG5, Q9UQN8, Q9UQP0, Q9Y2K6
    Related
    ENSP00000351122.1, ENST00000358355.5
    Conserved Domains (5) summary
    smart00695
    Location:810 → 895
    DUSP; Domain in ubiquitin-specific proteases
    cd02674
    Location:443 → 683
    Peptidase_C19R; A subfamily of peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...
    cl02553
    Location:144 → 291
    Peptidase_C19; Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyse bonds involving the carboxyl group of the C-terminal Gly ...
    pfam00443
    Location:144 → 682
    UCH; Ubiquitin carboxyl-terminal hydrolase
    pfam02148
    Location:30 → 90
    zf-UBP; Zn-finger in ubiquitin-hydrolases and other protein

  2. NM_001110303.4 → NP_001103773.2  ubiquitin carboxyl-terminal hydrolase 20

    See identical proteins and their annotated locations for NP_001103773.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) has a different 3' non-coding, terminal exon, compared to transcript variant 1. All three transcript variants encode the same protein.
    Source sequence(s)
    AL158207
    Consensus CDS
    CCDS43892.1
    UniProtKB/Swiss-Prot
    Q541F1, Q8IXQ1, Q96LG5, Q9UQN8, Q9UQP0, Q9Y2K6
    Related
    ENSP00000361506.3, ENST00000372429.8
    Conserved Domains (5) summary
    smart00695
    Location:810 → 895
    DUSP; Domain in ubiquitin-specific proteases
    cd02674
    Location:443 → 683
    Peptidase_C19R; A subfamily of peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...
    cl02553
    Location:144 → 291
    Peptidase_C19; Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyse bonds involving the carboxyl group of the C-terminal Gly ...
    pfam00443
    Location:144 → 682
    UCH; Ubiquitin carboxyl-terminal hydrolase
    pfam02148
    Location:30 → 90
    zf-UBP; Zn-finger in ubiquitin-hydrolases and other protein

  3. NM_006676.8 → NP_006667.3  ubiquitin carboxyl-terminal hydrolase 20

    See identical proteins and their annotated locations for NP_006667.3

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longest transcript. All three transcript variants encode the same protein.
    Source sequence(s)
    AL158207
    Consensus CDS
    CCDS43892.1
    UniProtKB/Swiss-Prot
    Q541F1, Q8IXQ1, Q96LG5, Q9UQN8, Q9UQP0, Q9Y2K6
    Related
    ENSP00000313811.4, ENST00000315480.9
    Conserved Domains (5) summary
    smart00695
    Location:810 → 895
    DUSP; Domain in ubiquitin-specific proteases
    cd02674
    Location:443 → 683
    Peptidase_C19R; A subfamily of peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...
    cl02553
    Location:144 → 291
    Peptidase_C19; Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyse bonds involving the carboxyl group of the C-terminal Gly ...
    pfam00443
    Location:144 → 682
    UCH; Ubiquitin carboxyl-terminal hydrolase
    pfam02148
    Location:30 → 90
    zf-UBP; Zn-finger in ubiquitin-hydrolases and other protein

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000009.12 Reference GRCh38.p14 Primary Assembly

    Range
    129835458..129881828
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060933.1 Alternate T2T-CHM13v2.0

    Range
    142040029..142086556
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9Y2K6.2 GenPept UniProtKB/Swiss-Prot:Q9Y2K6

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

Chemical Information
Molecular Biology Databases
Research Materials
Tools
Miscellaneous