Mylk myosin, light polypeptide kinase [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Mylkprovided by MGI
Official Full Name: myosin, light polypeptide kinaseprovided by MGI
Primary source: MGI:MGI:894806
See related: Ensembl:ENSMUSG00000022836 AllianceGenome:MGI:894806
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: KRP; Mlck; nmMlck; smMLCK; MLCK108; MLCK210; 9530072E15Rik; A930019C19Rik
Summary: Enables enzyme binding activity. Involved in tonic smooth muscle contraction. Acts upstream of or within cellular response to calcium ion; cellular response to potassium ion; and cellular response to xenobiotic stimulus. Located in cell-cell junction. Is expressed in several structures, including central nervous system; heart; liver; and retina. Human ortholog(s) of this gene implicated in adult respiratory distress syndrome; asthma; and thoracic aortic aneurysm. Orthologous to human MYLK (myosin light chain kinase). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in bladder adult (RPKM 454.5), large intestine adult (RPKM 64.4) and 8 other tissues See more
Orthologs: human all
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Genomic context

Location:: 16 B3; 16 24.49 cM

Exon count:: 36

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	16	NC_000082.7 (34565569..34822806)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	16	NC_000082.6 (34745199..35002436)

Chromosome 16 - NC_000082.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The SNHG12/microRNA-15b-5p/MYLK axis regulates vascular smooth muscle cell phenotype to affect intracranial aneurysm formation.
Title: The SNHG12/microRNA-15b-5p/MYLK axis regulates vascular smooth muscle cell phenotype to affect intracranial aneurysm formation.
Inhibition of non-muscular myosin light chain kinase accelerates the clearance of inflammatory cells by promoting the lysosome-mediated cell death.
Title: Inhibition of non-muscular myosin light chain kinase accelerates the clearance of inflammatory cells by promoting the lysosome-mediated cell death.
IGF2BP3-mediated enhanced stability of MYLK represses MSC adipogenesis and alleviates obesity and insulin resistance in HFD mice.
Title: IGF2BP3-mediated enhanced stability of MYLK represses MSC adipogenesis and alleviates obesity and insulin resistance in HFD mice.
MYOSIN LIGHT CHAIN KINASE DELETION WORSENS LUNG PERMEABILITY AND INCREASES MORTALITY IN PNEUMONIA-INDUCED SEPSIS.
Title: MYOSIN LIGHT CHAIN KINASE DELETION WORSENS LUNG PERMEABILITY AND INCREASES MORTALITY IN PNEUMONIA-INDUCED SEPSIS.
Tacrolimus-binding protein FKBP8 directs myosin light chain kinase-dependent barrier regulation and is a potential therapeutic target in Crohn's disease.
Title: Tacrolimus-binding protein FKBP8 directs myosin light chain kinase-dependent barrier regulation and is a potential therapeutic target in Crohn's disease.
Noncanonical function of long myosin light chain kinase in increasing ER-PM junctions and augmentation of SOCE.
Title: Noncanonical function of long myosin light chain kinase in increasing ER-PM junctions and augmentation of SOCE.
It was concluded that MLCK is part of a multicomponent signaling pathway in both the cytoplasm and nucleus that includes much more than simple regulation of conventional nonmuscle myosins through myosin regulatory light chain phosphorylation.
Title: CRISPR-Cas9/phosphoproteomics identifies multiple noncanonical targets of myosin light chain kinase.
nmMLCK is a potential molecular target to counteract early stages of atherosclerosis, such as endothelial dysfunction, inflammation through its capacity to increase transendothelial migration of monocytes and, most importantly, through the reorganization of VAMP-dependent vesicles associated with the release of IL-6 in modeled obstructive sleep apnea.
Title: Non-muscular myosin light chain kinase triggers intermittent hypoxia-induced interleukin-6 release, endothelial dysfunction and permeability.
Report direct role for hnRNPA1 in MYLK alternative splicing in lung endothelial cells.
Title: The Splicing Factor hnRNPA1 Regulates Alternate Splicing of the MYLK Gene.
a unique domain within the MLCK splice variant MLCK1 directs perijunctional actomyosin ring (PAMR) recruitment.
Title: Intracellular MLCK1 diversion reverses barrier loss to restore mucosal homeostasis.

Submit: New GeneRIF Correction See all GeneRIFs (49)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (6) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables actin binding	IEA Inferred from Electronic Annotation more info
enables calmodulin binding	IEA Inferred from Electronic Annotation more info
enables enzyme binding	IPI Inferred from Physical Interaction more info	PubMed
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables myosin light chain kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables myosin light chain kinase activity	ISO Inferred from Sequence Orthology more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in aorta smooth muscle tissue morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in bleb assembly	ISO Inferred from Sequence Orthology more info
involved_in cellular hypotonic response	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cellular response to calcium ion	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within cellular response to potassium ion	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within cellular response to xenobiotic stimulus	IDA Inferred from Direct Assay more info	PubMed
involved_in neurotransmitter receptor transport to postsynaptic membrane	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of calcium ion transport	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of wound healing	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in smooth muscle contraction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in tonic smooth muscle contraction	IBA Inferred from Biological aspect of Ancestor more info
involved_in tonic smooth muscle contraction	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in actin cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in cell projection	IEA Inferred from Electronic Annotation more info
located_in cell-cell junction	IDA Inferred from Direct Assay more info	PubMed
is_active_in cleavage furrow	IBA Inferred from Biological aspect of Ancestor more info
located_in cleavage furrow	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
is_active_in lamellipodium	IBA Inferred from Biological aspect of Ancestor more info
located_in lamellipodium	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in stress fiber	IBA Inferred from Biological aspect of Ancestor more info
located_in stress fiber	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: myosin light chain kinase, smooth muscle

Names: kinase-related protein; telokin

NP_001395190.1

EC 2.7.11.18

NP_001395191.1

EC 2.7.11.18

NP_001395192.1

EC 2.7.11.18

NP_647461.3

EC 2.7.11.18

XP_006521765.1

EC 2.7.11.18

XP_006521768.1

EC 2.7.11.18

XP_017172338.1

EC 2.7.11.18

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001408261.1 → NP_001395190.1 myosin light chain kinase, smooth muscle isoform 1

Status: VALIDATED

Source sequence(s)

AC110166, AC154813
NM_001408262.1 → NP_001395191.1 myosin light chain kinase, smooth muscle isoform 3

Status: VALIDATED

Source sequence(s)

AC110166, AC154813
NM_001408263.1 → NP_001395192.1 myosin light chain kinase, smooth muscle isoform 4

Status: VALIDATED

Source sequence(s)

AC110166, AC154813
NM_139300.4 → NP_647461.3 myosin light chain kinase, smooth muscle isoform 2

See identical proteins and their annotated locations for NP_647461.3

Status: VALIDATED

Source sequence(s)

AC110166, AC154813, CT010574

Consensus CDS

CCDS37320.1

UniProtKB/Swiss-Prot

Q3TSJ7, Q6PDN3, Q80UX0, Q80YN7, Q80YN8, Q8K026, Q924D2, Q9ERD3

UniProtKB/TrEMBL

B1B1A8

Related

ENSMUSP00000023538.9, ENSMUST00000023538.9

Conserved Domains (8) summary

cd00096
Location:637 → 706

Ig; Immunoglobulin domain

smart00408
Location:524 → 587

IGc2; Immunoglobulin C-2 Type

cd00063
Location:1362 → 1453

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

pfam07679
Location:1840 → 1930

I-set; Immunoglobulin I-set domain

cd14191
Location:1492 → 1750

STKc_MLCK1; Catalytic domain of the Serine/Threonine Kinase, Myosin Light Chain Kinase 1

pfam00069
Location:1495 → 1750

Pkinase; Protein kinase domain

pfam16620
Location:255 → 409

23ISL; Unstructured linker between I-set domains 2 and 3 on MYLCK

cl11960
Location:1269 → 1366

Ig; Immunoglobulin domain

RNA

NR_176622.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC110166, AC154813

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000082.7 Reference GRCm39 C57BL/6J

Range

34565569..34822806

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006521702.1 → XP_006521765.1 myosin light chain kinase, smooth muscle isoform X1

See identical proteins and their annotated locations for XP_006521765.1

UniProtKB/Swiss-Prot

Q3TSJ7, Q6PDN3, Q80UX0, Q80YN7, Q80YN8, Q8K026, Q924D2, Q9ERD3

Conserved Domains (5) summary

cd00063
Location:1353 → 1444

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

cd14191
Location:1483 → 1741

STKc_MLCK1; Catalytic domain of the Serine/Threonine Kinase, Myosin Light Chain Kinase 1

pfam07679
Location:1831 → 1921

I-set; Immunoglobulin I-set domain

pfam16620
Location:246 → 400

23ISL; Unstructured linker between I-set domains 2 and 3 on MYLCK

cl11960
Location:1260 → 1357

Ig; Immunoglobulin domain
XM_017316849.2 → XP_017172338.1 myosin light chain kinase, smooth muscle isoform X2

UniProtKB/Swiss-Prot

Q3TSJ7, Q6PDN3, Q80UX0, Q80YN7, Q80YN8, Q8K026, Q924D2, Q9ERD3

Conserved Domains (5) summary

cd00063
Location:1307 → 1398

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

cd14191
Location:1437 → 1695

STKc_MLCK1; Catalytic domain of the Serine/Threonine Kinase, Myosin Light Chain Kinase 1

pfam07679
Location:1785 → 1875

I-set; Immunoglobulin I-set domain

pfam16620
Location:246 → 400

23ISL; Unstructured linker between I-set domains 2 and 3 on MYLCK

cl11960
Location:1214 → 1311

Ig; Immunoglobulin domain
XM_006521705.3 → XP_006521768.1 myosin light chain kinase, smooth muscle isoform X3

Conserved Domains (4) summary

cd05762
Location:38 → 135

Ig8_hMLCK_like; Eighth immunoglobulin (Ig)-like domain of human myosin light-chain kinase (MLCK) and similar domains

cd00063
Location:131 → 222

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

pfam07679
Location:609 → 699

I-set; Immunoglobulin I-set domain

cl21453
Location:261 → 519

PKc_like; Protein Kinases, catalytic domain