Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 3.86348 | 1.13583 | 3.93599 | 1.01636 | |
| 10day-seedling | 2.9636 | 0.376843 | 4.77176 | 0.20624 | |
| 14day-seedling | 2.70145 | 0 | 4.7016 | 0 | |
| 25day-aerial | 2.64941 | 0.838518 | 4.41389 | 0.840959 | |
| 35day-aerial | 3.04935 | 0.469215 | 4.81637 | 0.378472 | |
| 42day-rosette | 2.51974 | 0.785415 | 4.43682 | 0.989447 | 42day-stem | 3.37658 | 0.140474 | 5.10194 | 0.085325 |
| 53day-rosette | 1.91362 | 0.582597 | 3.75612 | 0.593468 | 53day-stem | 2.8636 | 0.383433 | 4.28107 | 0.80397 | 53day-inflores | 3.62065 | 0.188697 | 5.04469 | 0.243301 | 53day-silique | 4.04496 | 0.164064 | 5.18756 | 0.153365 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 35.34 | 11.48 | 3.49e-4 | -3.46 | 11.4 | 3.71e-4 | -3.43 |
| 2 | 1.000 | 34.21 | 6.23 | 1.33e-2 | -1.87 | 6.36 | 1.22e-2 | -1.92 | |
| 3 | 1.000 | 37.82 | 13.55 | 8.36e-5 | -4.08 | 12.78 | 1.43e-4 | -3.85 | |
| 10day-seedling | 1 | 1.000 | 25.64 | 12.78 | 1.42e-4 | -3.85 | 7.29 | 6.40e-3 | -2.19 |
| 2 | 1.000 | 25.84 | 12.59 | 1.62e-4 | -3.79 | 6.78 | 9.09e-3 | -2.04 | |
| 3 | 1.000 | 26.22 | 14.85 | 3.39e-5 | -4.47 | 8.14 | 3.55e-3 | -2.45 | |
| 14day-seedling | 1 | 0.998 | 27.85 | 14.28 | 5.03e-5 | -4.3 | 7.64 | 5.03e-3 | -2.3 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 25day-aerial | 1 | 1.000 | 30.39 | 17.53 | 5.27e-6 | -5.28 | 11.78 | 2.85e-4 | -3.54 |
| 2 | 1.000 | 27.73 | 13.69 | 7.57e-5 | -4.12 | 7.45 | 5.74e-3 | -2.24 | |
| 3 | 0.999 | 25.99 | 12.11 | 2.26e-4 | -3.65 | 6.55 | 1.07e-2 | -1.97 | |
| 35day-aerial | 1 | 1.000 | 25.8 | 11.34 | 3.85e-4 | -3.41 | 5.8 | 1.79e-2 | -1.75 |
| 2 | 0.999 | 26.52 | 13.8 | 7.01e-5 | -4.15 | 7.67 | 4.92e-3 | -2.31 | |
| 3 | 0.992 | 27.42 | 14.23 | 5.21e-5 | -4.28 | 8.04 | 3.79e-3 | -2.42 | |
| 42day-rosette | 1 | 1.000 | 26.29 | 12.75 | 1.45e-4 | -3.84 | 5.64 | 2.00e-2 | -1.7 |
| 2 | 0.998 | 32.17 | 17.79 | 4.41e-6 | -5.36 | 12.1 | 2.28e-4 | -3.64 | |
| 3 | 1.000 | 26.89 | 13.37 | 9.45e-5 | -4.02 | 7.8 | 4.48e-3 | -2.35 | |
| 42day-stem | 1 | 0.999 | 23.21 | 11.52 | 3.41e-1 | -3.47 | 6.27 | 1.35e-1 | -1.87 |
| 2 | 0.998 | 24.72 | 12.17 | 2.16e-1 | -3.66 | 6.62 | 1.13e-1 | -1.99 | |
| 3 | 0.999 | 25.77 | 12.39 | 1.86e-1 | -3.74 | 6.85 | 1.47e-1 | -1.83 | |
| 53day-rosette | 1 | 1.000 | 28.75 | 14.7 | 3.75e-5 | -4.43 | 8.71 | 2.38e-3 | -2.62 |
| 2 | 1.000 | 30.64 | 18.36 | 2.98e-6 | -5.53 | 12.64 | 1.57e-4 | -3.81 | |
| 3 | 1.000 | 30.52 | 17.63 | 4.92e-6 | -5.31 | 10.98 | 4.97e-4 | -3.3 | |
| 53day-stem | 1 | 1.000 | 27.1 | 13.68 | 7.63e-5 | -4.12 | 7.16 | 6.99e-3 | -2.16 |
| 2 | 1.000 | 27.27 | 12.5 | 1.73e-4 | -3.76 | 7.85 | 4.35e-3 | -2.36 | |
| 3 | 0.999 | 32.65 | 15.04 | 2.96e-5 | -4.53 | 12.09 | 2.29e-4 | -3.64 | |
| 53day-inflores | 1 | 0.990 | 24.57 | 11.18 | 4.32e-4 | -3.36 | 6.02 | 1.54e-2 | -1.81 |
| 2 | 0.999 | 26.51 | 11.88 | 2.66e-4 | -3.58 | 7.32 | 6.26e-3 | -2.2 | |
| 3 | 0.991 | 24.45 | 10.63 | 6.33e-4 | -3.2 | 6.04 | 1.52e-2 | -1.82 | |
| 53day-silique | 1 | 1.000 | 29.84 | 9.99 | 9.81e-4 | -3.01 | 6.04 | 1.52e-2 | -1.82 |
| 2 | 1.000 | 28.5 | 9.2 | 1.69e-3 | -2.77 | 5.5 | 2.21e-2 | -1.66 | |
| 3 | 1.000 | 29.22 | 10.25 | 8.21e-4 | -3.09 | 6.52 | 1.09e-2 | -1.96 | |
NGS expression data
Expression of miR165a transcript in NGS dataSequences
miRNA transcript
CUGCUAAGAUCGAUUAUCAUGAGGGUUAAGCUAUUUCAGUUGAGGGGAAUGUUGUCUGGAUCGAGGAUAUUAUAGAUAUAUACAUGUGUAUGUUAAUGAUUCAAGUGAUCAUAGAGAGUAUCCUCGGACCAGGCUUCAUCCCCCCCAACAUGUUAUUGCCUCUGAUCACCAUUUAUUGUUACAUUUUUUUUUGUUAAUUACUUGCGCAAAUUACAAAAGCUUGGUUUUUGUGAUGACUUUGAAUC
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UCGGACCAGGCUUCAUCCCCC | miRNA | 124 | 144 | |
TGCTAAGATCGATTATCATGAGGGTTA | primer | 5p | 2 | 28 |
TTCAAAGTCATCACAAAAACCAAGC | primer | 3p | 219 | 243 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR165a found in PubMed |