Switch to: Actin
Expression Profile Reference: pp2a
The expression values are rescaled. Read more
Expression Data
Development stage actin PP2A Log SD Log SD seed 3.86348 1.13583 3.93599 1.01636 10day-seedling 2.9636 0.376843 4.77176 0.20624 14day-seedling 2.70145 0 4.7016 0 25day-aerial 2.64941 0.838518 4.41389 0.840959 35day-aerial 3.04935 0.469215 4.81637 0.378472 42day-rosette 2.51974 0.785415 4.43682 0.989447 42day-stem 3.37658 0.140474 5.10194 0.085325 53day-rosette 1.91362 0.582597 3.75612 0.593468 53day-stem 2.8636 0.383433 4.28107 0.80397 53day-inflores 3.62065 0.188697 5.04469 0.243301 53day-silique 4.04496 0.164064 5.18756 0.153365
Raw Data
Development stage rep R2 Ct actin PP2A ΔCt 2^(-ΔCt) log10 ΔCt 2^(-ΔCt) log10 seed 1 1.000 35.34 11.48 3.49e-4 -3.46 11.4 3.71e-4 -3.43 2 1.000 34.21 6.23 1.33e-2 -1.87 6.36 1.22e-2 -1.92 3 1.000 37.82 13.55 8.36e-5 -4.08 12.78 1.43e-4 -3.85 10day-seedling 1 1.000 25.64 12.78 1.42e-4 -3.85 7.29 6.40e-3 -2.19 2 1.000 25.84 12.59 1.62e-4 -3.79 6.78 9.09e-3 -2.04 3 1.000 26.22 14.85 3.39e-5 -4.47 8.14 3.55e-3 -2.45 14day-seedling 1 0.998 27.85 14.28 5.03e-5 -4.3 7.64 5.03e-3 -2.3 2 - - - - N/D - - N/D 3 - - - - N/D - - N/D 25day-aerial 1 1.000 30.39 17.53 5.27e-6 -5.28 11.78 2.85e-4 -3.54 2 1.000 27.73 13.69 7.57e-5 -4.12 7.45 5.74e-3 -2.24 3 0.999 25.99 12.11 2.26e-4 -3.65 6.55 1.07e-2 -1.97 35day-aerial 1 1.000 25.8 11.34 3.85e-4 -3.41 5.8 1.79e-2 -1.75 2 0.999 26.52 13.8 7.01e-5 -4.15 7.67 4.92e-3 -2.31 3 0.992 27.42 14.23 5.21e-5 -4.28 8.04 3.79e-3 -2.42 42day-rosette 1 1.000 26.29 12.75 1.45e-4 -3.84 5.64 2.00e-2 -1.7 2 0.998 32.17 17.79 4.41e-6 -5.36 12.1 2.28e-4 -3.64 3 1.000 26.89 13.37 9.45e-5 -4.02 7.8 4.48e-3 -2.35 42day-stem 1 0.999 23.21 11.52 3.41e-1 -3.47 6.27 1.35e-1 -1.87 2 0.998 24.72 12.17 2.16e-1 -3.66 6.62 1.13e-1 -1.99 3 0.999 25.77 12.39 1.86e-1 -3.74 6.85 1.47e-1 -1.83 53day-rosette 1 1.000 28.75 14.7 3.75e-5 -4.43 8.71 2.38e-3 -2.62 2 1.000 30.64 18.36 2.98e-6 -5.53 12.64 1.57e-4 -3.81 3 1.000 30.52 17.63 4.92e-6 -5.31 10.98 4.97e-4 -3.3 53day-stem 1 1.000 27.1 13.68 7.63e-5 -4.12 7.16 6.99e-3 -2.16 2 1.000 27.27 12.5 1.73e-4 -3.76 7.85 4.35e-3 -2.36 3 0.999 32.65 15.04 2.96e-5 -4.53 12.09 2.29e-4 -3.64 53day-inflores 1 0.990 24.57 11.18 4.32e-4 -3.36 6.02 1.54e-2 -1.81 2 0.999 26.51 11.88 2.66e-4 -3.58 7.32 6.26e-3 -2.2 3 0.991 24.45 10.63 6.33e-4 -3.2 6.04 1.52e-2 -1.82 53day-silique 1 1.000 29.84 9.99 9.81e-4 -3.01 6.04 1.52e-2 -1.82 2 1.000 28.5 9.2 1.69e-3 -2.77 5.5 2.21e-2 -1.66 3 1.000 29.22 10.25 8.21e-4 -3.09 6.52 1.09e-2 -1.96
Expression of miR165a transcript in NGS data CUGCUAAGAUCGAUUAUCAUGAGGGUUAAGCUAUUUCAGUUGAGGGGAAUGUUGUCUGGAUCGAGGAUAUUAUAGAUAUAUACAUGUGUAUGUUAAUGAUUCAAGUGAUCAUAGAGAGUAUCCUCGGACCAGGCUUCAUCCCCCCCAACAUGUUAUUGCCUCUGAUCACCAUUUAUUGUUACAUUUUUUUUUGUUAAUUACUUGCGCAAAUUACAAAAGCUUGGUUUUUGUGAUGACUUUGAAUC
sequence type arm start end UCGGACCAGGCUUCAUCCCCC
miRNA 124 144 TGCTAAGATCGATTATCATGAGGGTTA
primer 5p 2 28 TTCAAAGTCATCACAAAAACCAAGC
primer 3p 219 243
Length = 245 nt MFE = -73.10 kcal/mol