JCRB1355 1-1ras1000
Cell information
Important Notice(s)Balb/c 3T3 A31-1に派生する細胞株の由来の問題について
The problem of origin of Balb/c 3T3 A31-1 derived cell lines.
Cell type:general cells (View Pricing Information)
JCRB No. | JCRB1355 | Cell Name | 1-1ras1000 |
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Profile | The ras-transformed BALB/c 3T3 A31-1-1variant cell lines were strongly tumorinenic, but the cells were not metastatic. (Strain problem was found. Refer Important Notice at cell detail page.) | Other Name | |
Animal | mouse | Strain | Swiss |
Genus | Mus | Species | musculus |
Sex | Age | embryo | |
Identity | Tissue for Primary Cancer | embryo | |
Case history | normal | Metastasis | No |
Tissue Metastasized | Genetics | Origin of this cell line, Balb/c 3T3 A31-1, was found not to be derived from BALB 3T3 A31. | |
Life Span | infinite | Crisis PDL | |
Morphology | fibroblast-like | Character | Fibroblast, ras-transformed subclone of Balb/3T3 A31-1-1. |
Classify | transformed | Established by | Tatsuka, M. |
Registered by | Ota, T. | Regulation for Distribution | |
Comment | Year | 2008 | |
Medium | Eagle's minimal essential medium with 10% fetal calf serum | Methods for Passages | 0.25% trypsin & 0.02% EDTA. Practically, 1/100 dilution once a week. Avoid to be confluent. |
Cell Number on Passage | --- | Race | |
CO2 Conc. | 5% | Tissue Sampling | embryo, whole |
Tissue Type |
Detection of virus genome fragment by Real-time PCR | |||||||||
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Detected DNA Virus | not tested | Detected RNA Virus | not tested |
Reference | |
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Pubmed id:9096670 | A BALB/c 3T3-transformed cell line suitable for transfection assay of metastasis-inducing genes. Tatsuka M,Ota T,Maeda M,Wada M,Yamagishi N,Taniguchi S,Seiki M,Odashima S Int J Cancer. 1997 Mar 28;71(1):88-93 |
Pubmed id:7394516 | Cell variants showing differential susceptibility to ultraviolet light--induced transformation. Kakunaga T,Crow JD Science. 1980 Jul 25;209(4455):505-7 |
Pubmed id:4311790 | Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells. Jainchill JL,Aaronson SA,Todaro GJ J Virol. 1969 Nov;4(5):549-53 |
Pubmed id:4301006 | Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40. Aaronson SA,Todaro GJ J Cell Physiol. 1968 Oct;72(2):141-8 |
Pubmed id:13985244 | Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. TODARO GJ,GREEN H J Cell Biol. 1963 May;17():299-313 |
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