has curated information from 8 experiments about cydA

Gene search
Gene name ECK no. Synonyms Function
cydA
 ECK0721 b0733, Cytochrome d (bd-I) terminal oxidase subunit I
Multifun Aerobic respiration. Electron acceptor. Membrane. Inner membrane.
Comments  EcoGene suggests alternate start codon
Genome location
 Minutes Kilobases Transcription unit Feature Type Length MW predicted (Da) Multifun
770681
772249
 16.63
771.465
 TU00100 Protein 1572 58336.52 MultiFun
← farR
hrsA →
ybgG →
cydA →
cydB →
ybgT →
ybgE →

Current literature
(EcoGene)

EG10173 EG10173 1786953 CB006163 EG10173 b0733 ECK0721 P11026 JW0722

Integral Membrane Protein
Comments Multiple transmembrane helices predicted by HMMTOP and TMHMM, no signal peptide predicted.

Proteomics

 Proteomic study of E. coli to examine detection of essential and horizontally transferred gene products [ Full annotation of this paper]
Taoka M et al. Only a small subset of the horizontally transferred chromosomal genes in Escherichia coli are translated into proteins.
Mol Cell Proteomics. 2004 3(8):780-7
PMF (Number of peptides): 37

Proteomics

 Identification of 1132 expressed proteins from Tryptic digest of all E. coli proteins [ Full annotation of this paper]
Corbin RW et al. Toward a protein profile of Escherichia coli: comparison to its transcription profile
Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9232-7
PMF (Number of peptides): 4 Predicted Number of tryptic peptides: 15

Proteomics

 Global topology analysis of inner membrane protiens using PhoA and Gfp fusions. [ Full annotation of this paper]
Daley DO et al. Global topology analysis of the Escherichia coli inner membrane proteome.
Science. 2005 May 27;308(5726):1321-3.
PhoA activity: 0 GFP activity: 0.94 C terminal location: in

Microarray Global identification of members in FNR regulation as E. coli adapts from aerobic to anaerobic growth using microarray technologies. [Full annotation of this paper]
Constantinidou C et al. A reassessment of the FNR regulon and transcriptomic analysis of the effects of nitrate, nitrite, NarXL and NarQP as Escherichia coli K-12 adapts from aerobic to anaerobic growth.
J Biol Chem. 2005 Dec 22
Fold induction 0.8 Aim of Research Aerobic vs. anaerobic induction of the FNR regulon
Fold induction 0.8 Aim of Research Aerobic fnr vs. anaerobic induction of the FNR regulon
Fold induction 3 Aim of Research Anaerobic fnr vs. anaerobic induction of the FNR regulon
Fold induction 0.9 Aim of Research Anaerobic + NO3- vs. anaerobic induction of the FNR regulon
Fold induction 3.2 Aim of Research Anaerobic fnr + NO3- vs. anaerobic induction of the FNR regulon
Fold induction 1.3 Aim of Research Anaerobic + NO2- vs. anaerobic induction of the FNR regulon
Fold induction 3.7 Aim of Research Anaerobic fnr + NO2- vs. anaerobic induction of the FNR regulon

Transcript Level Identification of 2881 transcripts from the E. coli genome [Full annotation of this paper]
Corbin RW et al. Toward a protein profile of Escherichia coli: comparison to its transcription profile
Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9232-7
Transcript level 3720 Aim of Research Identification of expressed genes in E coli by measuring RNA signal intensity

Bioinformatic Calculation of the conservation of a gene across 33 microbial species [Full annotation of this paper]
Gerdes SY et al. Experimental determination and system level analysis of essential genes in Escherichia coli MG1655
J Bacteriol. 2003 Oct;185(19):5673-84
Description This number is the fraction of times this gene occurs in 33 different microbial genomes, called the evolutionary retention index (ERI) by the authors. The higher the number the more conserved this gene is in these different genomes, with a score of 1 meaning it is present in all 33 genomes (33/33=1).
ERI Score 0.625

Bioinformatic Gene knockouts obtained in essential genes [Full annotation of this paper]
Kang Y et al. Systematic mutagenesis of the Escherichia coli genome.
Bacteriol. 2004 Aug;186(15):4921-30
Description Potentially essential gene in which Sce-poson insertion was obtained
Sce Poson insertion site into gene (bp) 568

Genetics Identification of essential genes in E. coli using transposon footprinting [Full annotation of this paper]
Gerdes SY et al. Experimental determination and system level analysis of essential genes in Escherichia coli MG1655
J Bacteriol. 2003 Oct;185(19):5673-84
Gene disrupted EB0170 Phenotype Essential gene

Annotation History
Data checked by Richard Horler Last Update 2004/09/14 EB number EB0170