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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:24801*  Cite 
Description

Structural differentiation of actin network in lamellipodium. Electron micrograph of Xenopus fibroblasts after regular extraction in the presence of polyethelene glycol (PEG) and phalloidin. While the actin network in the front zone of these cels remains dense, the actin network at the lamellipodial rear disassembled in the course of unprotected extraction (CIL 24800). Image corresponds to Figure 6c from J Cell Biol. 1999 May 31;145(5):1009-26.

Technical Details

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Biological Sources
NCBI Organism Classification
Xenopus laevis
Cell Type
fibroblast
Cellular Component
lamellipodium
actin cytoskeleton
filopodium
Biological Context
Biological Process
cellular localization
actin filament organization
Molecular Function
structural constituent of cytoskeleton
Attribution
Names
Tatyana M. Svitkina
Gary G. Borisy
Published
J Cell Biol. 1999 May 31;145(5):1009-26.
Pubmed
10352018
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL24801
Archival Resource Key (ARK)
ark:/b7295/w9cil24801
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
optical path length gradient
Source of Contrast
differences in deposition of metal shadow
Visualization Methods
shadowing and plating
platinum replica
Processing History
unprocessed raw data
Sample Preparation
Methods
permeabilized tissue
glutaraldehyde fixed tissue
phalloidin
polyethelene glycol
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 4380px ——
Y 3360px ——