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CABRI:DSMZ_MUTZ |
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Accession_number |
ACC 291
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Cell_line_name |
U-138-MG
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Brief_description |
confirmed as human with IEF of AST, LDH, MDH, NP established from the
glioblastoma of a 47-year-old Caucasian man
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Description |
human glioblastoma established from the glioblastoma of a 47-year-old Caucasian
man confirmed as human with IEF of AST, LDH, MDH, NP
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Depositor |
obtained from DKFZ Tumorbank, Heidelberg, Germany
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Morphology |
fibroblastoid fusiform cells growing as mono- or multilayers
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Culture_conditions |
90% Ham's F10 + 10% FBS split confluent culture 1:5 to 1:10 every 4-8 days using
trypsin/EDTA; seed out at about 0.1-0.2 x 105 cells/cm 2 at 37C with 5% CO2 cell
harvest of ca. 0.5-0.7 x 105 /cm 2
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Viruses |
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV -,
HTLV-I/II -, SMRV -
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Properties |
cytokeratin -, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
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Release_conditions |
-
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Hazard |
-
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Storage |
frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 106 cells/ampoule
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Doubling_time |
ca. 70 hours
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Mycoplasma |
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
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Fingerprint |
multiplex PCR of minisatellite markers revealed a unique DNA profile
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Cytogenetics |
human flat-moded hypotriploid karyotype with 20% polyploidy - 60(57-63)<3n>XYY, -
3, -5, -6, -8, -9, -10, -11, -13, -14, -17, -19, +2mar, add(4)(p15), del(6)(q15),
dup(7)(q35q21), add(11)(p15), add(12)(p11), add(14)(q32)
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Bibliographic_reference |
Ponten et al., Acta Pathol Microbiol Scand, 74: 1201 [PMID: 4313504
] |
Beckman et al., Hum Hered, 21: 1201 (1971) [PMID: 4332744]
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